Gray leaf spot [GLS, causal agent Cercospora zeae-maydis (Tehon and E. Y. Daniels)] is an important fungal disease of maize in the U.S. and worldwide. The IBM population, an advanced intercross recombinant inbred line population derived from a cross between the maize lines Mo17 (resistant) and B73 (susceptible), was evaluated in three environments (Andrews, NC in 2005, 2006, and 2007) for resistance to GLS and for days from planting to anthesis (DTA). A conventional recombinant inbred line population derived from the same two parents (the “Stuber” population) was also assessed for GLS resistance in two environments (Andrews NC, 2004 and 2005). Quantitative trait loci (QTL) for GLS resistance were detected in each population. Five significant QTL were detected in the IBM population in bins 1.05, 2.04, 4.05, 9.03, and 9.05. In each case the QTL were localized to regions less than 3 centiMorgans (cM). Two QTL for GLS resistance were identified in the Stuber population in bins 2.04 and 7.05. The GLS QTL in bin 2.04 was previously identified as a QTL for southern leaf blight resistance in the IBM population. These results were compared with results from five previous GLS QTL studies and two potential GLS QTL “hotspots” were identified in bins 1.05???1.06 and 2.03???2.05. As expected, QTL were identified with much more precision in the IBM population compared to the Stuber population and to previous studies. There was no significant correlation between disease resistance and days to anthesis. Three DTA QTL were detected in bins 4.09, 8.05, and 9.02, which did not co-localize with GLS QTL.