Youth networks and organizations of young farmers, producers and entrepreneurs play a central role as partners in development initiatives that target youth in rural and agrifood spaces. By understanding and applying gender‑transformative and socially inclusive approaches, youth organizations can become more equitable, accessible, impactful and pluralistic platforms that legitimately represent the heterogeneity of young people and amplify the voices of those most marginalized or those in situations of vulnerability. These guidelines are a practical tool for youth organizations to better understand gender equality and social inclusion (GESI) and their importance, offering a step-by-step methodology to self-assess main gaps and areas for improvement as the basis to embed inclusive approaches in their organizational practice.