Seven rhabdoviruses isolated from fish suffering from epizootic ulcerative syndrome (EUS) were compared in terms of their morphology, cytopathogenicity, antigenic relatedness and structural polypeptide composition. All strains exhibited a bullet-shaped morphology, but the T9204 isolate was found to be longer and more variable in length than the other strains. Sixteen fish cell lines investigated showed some variation in susceptibility to each isolate, but the cytopathic changes induced by T9204 in SSN-2, RSN, GCP, ONP, FHM, AS and MUL lines were significantly different from the other isolates. Polyclonal antisera raised against the BPV, 20E and SL11 strains neutralized six isolates (BPV, 02, 19, 20E, A4 and SL11), but not T9204. Conversely, anti-T9204 serum only neutralized homologous virus. Polyacrylamide gel electrophoresis demonstrated that BPV, 02, 19, 20E, A4 and SL11 had virtually identical protein profiles, whereas T9204 differed both in the number of protein bands and in their migration pattern. Western blots of these gels identified the proteins specific to T9204 that reacted with anti-T9204 serum. Therefore, the isolates represent two distinct species of fish rhabdoviruses, but as yet, no causal relationship with EUS, or any other disease condition, has been established.