Jembrana disease virus (JDV) causes a potentially fatal infection of Bali cattle that is endemic on the island of Bali. This project developed a cell line expressing JDV tat protein that may stimulate virus growth in culture, but which has ey to be infected with JDV. It also developed a robust in vitro test which will detect recombinant JDV protein. The potential for this assay to detect viral antigen in samples from infected cattle is very high.